Journal: bioRxiv

Article Title: Oral immunization with rVSV bivalent vaccine elicits protective immune responses, including ADCC, against both SARS-CoV-2 and Influenza A viruses

doi: 10.1101/2023.07.14.549076

Figure Lengend Snippet: A) The duration of anti-SARS-CoV-2 RBD (endpoint titers) in the i.n. immunized mice sera after booster immunization (3wpB and 5wpB) were measured. B) The neutralization titers (50% inhibitory dose, ID 50 ) in immunized mice sera (3wpB) against pseudovirus PV-Luc-SP Delta infection were determined. The serially diluted mouse sera were incubated with PV-Luc-SP Delta (∼10 4 RLU) and then, the mixtures (PV + Sera) were used to inoculate A549 ACE2 cells. The infection of PV was determined by luciferase assay at 48∼66 hrs post-infection. The percentage of infection was calculated compared with no serum control. The 50% inhibition dose (ID 50 ) neutralizing Ab titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. C) The duration of neutralizing Ab ID 50 titers in the i.n. immunized mice sera collected at 3wpB and 5wpB. Data represent mean ±SEM. Statistical significance was determined using one-way ANOVA test and Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. wpB, week post Boost.

Article Snippet: The ID 50 was calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0.

Techniques: Neutralization, Infection, Incubation, Luciferase, Inhibition

Journal: Clinical & Translational Immunology

Article Title: Assessment of antibodies in the upper and lower human respiratory tract at steady state and after respiratory viral infection

doi: 10.1002/cti2.1460

Figure Lengend Snippet: Detection of antibodies in BAL samples and nasopharyngeal swabs. (a) ELISA curves from plasma, NP swab and BAL for total Ig (heavy and light chain), IgG, IgA and IgM. Measured optical density at 490 nm and a fitted sigmoidal 4 parameter logistic curves are shown. Each curve represents a different subject. The dotted horizontal line represents the cut‐off value for endpoint titre calculations (3× background signal). (b) Interpolated concentrations of IgG, IgA and IgM for paired plasma, NP swab and BAL samples. (c) Ratios of IgG and IgA concentrations in paired samples. Samples from control subjects were used for this figure ( n = 8). Statistical significance was determined by a Friedman's test with Dunn's correction for multiple comparisons.

Article Snippet: Concentrations were interpolated from a standard curve using a sigmoid 4PL curve in Graphpad Prism v9.

Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Control

Journal: Scientific Reports

Article Title: Membrane estrogen receptor α signaling modulates the sensitivity to estradiol treatment in a dose- and tissue- dependent manner

doi: 10.1038/s41598-023-36146-9

Figure Lengend Snippet: Loss of mERα signaling causes a right shift of E2 treatment responses. Tissue responses to different doses of E2 treatment in uterus ( A ), thymus ( B ), gonadal fat ( C ), tibia trabecular bone ( D ), and tibia cortical bone ( E ). X-axis represents the logarithmic mean value of serum E2 concentrations at termination (pg/mL). Y-axis represents the % response to E2 treatment calculated from % E2 response in WT mice in the high-dose experiment, which was set to 100%. All other % E2 responses were related to the % E2 response in WT mice from the high-dose experiment. The E2 response curve is visualized by using a sigmoidal, four parameter logistic (4PL) curve fitting model (GraphPad). Analyses of differences in E2 responses between WT and C451A were done by two-way ANOVA. All individual values are presented with mean (horizontal line) and SEM (vertical lines). ** p < 0.01, *** p < 0.001, conc.; concentration, BV/TV; bone volume per tissue volume, Ct. Th.; cortical thickness.

Article Snippet: The E2 response curve is visualized by using a sigmoidal, four parameter logistic (4PL) curve fitting model (GraphPad).

Techniques: Concentration Assay